rabbit polyclonal anti smox  (Proteintech)

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: Comparison of clinicopathological factors and SMOX expression in patients with CRC.

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Comparison, Expressing

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: Expression of SMOX in colorectal cancer (CRC) cell lines and the effects of SMOX siRNA (small interfering RNA). SMOX expression and inhibition were confirmed by RT-PCR and immunoblot. ( A ) Screening by RT-PCR of SMOX in CRC cell lines and confirmation of SMOX inhibition compared with control cells. ( B ) Relative RNA expression of SMOX. The expression of SMOX siRNA-transfected cell lines was significantly lower than that in the control cell lines ( *** p < 0.001). ( C ) Expression levels of SMOX and β-actin were determined by Western blotting in CRC cell lines. ( D ) Relative protein levels of SMOX. SMOX siRNA-transfected cell expression levels were lower than those in the control (*** p < 0.001).

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Expressing, Small Interfering RNA, Inhibition, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, RNA Expression, Transfection

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: The results of the transwell assay regarding metastasis. Reduced expression of SMOX inhibits the migration and invasion abilities of CRC cells. The result of the chamber migration assay and invasion assay. Cells were visualized in violet color. ( A ) Migration of the siRNA treatment group was reduced in comparison with the control group. The images of the cells were on the bottom membrane (Original magnification ×40). ( B ) siRNA-treated cells were less numerous than control cells. The images of the cells were on the bottom membrane (Original magnification ×40). ( C ) The graph indicates the percentage of cells and the results of the chamber migration assay (HCT116, 35%; HT29, 46%; SW480, 34%; and SW620, 22%) (* p < 0.05, *** p < 0.001). ( D ) The graph expresses the percentage of cells in the chamber invasion assay (HCT116, 31%; HT29, 41%; SW480, 35%; and SW620, 18%) (** p < 0.01, *** p < 0.001).

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Transwell Assay, Expressing, Migration, Invasion Assay, Comparison, Control, Membrane

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: The result of thee semisolid agar colony-forming assay. Anchorage-independent growth in soft agar was examined with control cells and cells that displayed downregulation of SMOX expression. The numbers of colonies were measured after 14 days. Colony formation in the siRNA-treated group was reduced compared to that in the control group (HCT116, 31%; HT29, 38%; SW480, 33%; and SW620, 21%). Colonies were visualized in violet color. ( A ) Comparison of the growth of colonies with control cells and siRNA-treated cells (Original magnification ×40). ( B ) The graph indicates the number of colonies and the percentage difference between the control cells and siRNA-treated cells (* p < 0.05, *** p < 0.001).

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Control, Expressing, Comparison

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: The result of IHC for SMOX expression in CRC ( A – D ). In total, 350 specimens were used, which were scored by multiplying the staining frequency and staining intensity. Low/High expression of SMOX was discovered in 182/168 CRC tissues. The Kaplan–Meier survival analysis was performed for all the sampled CRC patients, based on numerous variables and their expression of SMOX. SMOX proteins and nuclei were visualized by brown and violet, respectively. ( A ) Normal tissue without expression of SMOX (Original magnification ×200). ( B ) Cancer tissue without expression of SMOX (Original magnification ×200). ( C ) Cancer tissue with low expression of SMOX (Original magnification ×200). ( D ) Cancer tissue with high expression of SMOX (Original magnification ×200). ( E ) SMOX expression and overall survival of colon cancer patients were related. Following the expression of SMOX, patients were divided into two groups and the survival rate was determined using a Kaplan-Meier analysis ( p = 0.001).

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Expressing, Staining

Journal: Biomedicines

Article Title: Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

doi: 10.3390/biomedicines10030626

Figure Lengend Snippet: Cox regression analysis of the clinicopathological factors in CRC.

Article Snippet: The anti-human primary rabbit polyclonal SMOX antibody (Proteintech, Rosemont, IL, USA) was diluted at 1:500, and the membrane was subsequently incubated with the antibody in BSA at 4 °C overnight.

Techniques: Expressing